Eye Moisturizing, Anti-Light Damage Peptides

Corneal Repair Trial for Eyes

Chronic Dry Eye Syndrome

The Result1

Hydrochloric acid caused a second-degree (mild to moderate) injury to the rabbit eyes, presenting as redness (indicative of epithelial cell death) and dryness. However, in the treatment group following the use of PROTAI™, observation after 8 hours revealed that the corneal condition remained good, even superior to the control group. This demonstrates the significant corneal cell repair effects of PROTAI™. Furthermore, based on the observation of eye moisture levels, the sequence-specific peptide of PROTAI™ indeed possesses moisturizing properties, providing protective effects during the acute phase of dry eye syndrome.

The Result2

8 days after the cessation of using eye drops, the eye moisture level increased by 40-50% compared to the damage group. This result indeed demonstrates the significant effectiveness of the sequence-specific peptide of PROTAI™ in improving dry eye syndrome and its ability to achieve long-term improvement.

Anti-Glare Experiment

The widespread use of 3C products (computers, communication devices, and consumer electronics) and the increasing prevalence of light pollution in daily life may accelerate eye aging. PROTAI™ has been experimentally verified for its efficacy in preventing damage to eye cells caused by high-energy ultraviolet radiation, blue light exposure, and oxidative stress.

3C products and unfavorable light sources are both reasons why modern individuals are susceptible to light pollution. Due to the excessive blue light emitted by electronic devices, it can penetrate the crystalline lens and reach the retina. Prolonged exposure to blue light may lead to the generation of free radicals in the photoreceptor cells. These free radicals accelerate the oxidation processes in the retinal epithelial cells and photoreceptor cells in the macula. This oxidative action can damage retinal cells and may eventually lead to macular degeneration. In the early stages, this could cause symptoms such as eye irritation, and photophobia, and in severe cases, it may lead to blindness.

ARPE-19 Cell

5x10⁵ cells were seeded in a 24-well culture plate, and PROTAI™ was added. After 24 hours of cultivation, the cells were exposed to UV light at 100 millijoules (mJ) using a UV lightbox. Subsequently, the culture plate was left in the UV lightbox for 30 minutes. Following this, the supernatant was extracted for the MTT cell viability assay.

 

 

 

 

The Result

After conducting a comparison with another group that similarly received PROTAI™ sequence-specific peptide along with UV exposure, we observed a 49% increase in the survival rate of ARPE-19 cells.